Single-Leaf Resolution of the Temporal Population Dynamics of Aureobasidium pullulans on Apple Leaves
نویسندگان
چکیده
منابع مشابه
Role of microbial immigration in the colonization of apple leaves by Aureobasidium pullulans.
The role of microbial immigration in the veinal colonization pattern of Aureobasidium pullulans on the adaxial surface of apple leaves was investigated in two experiments at two periods (early and late seasons) in 2004 by applying green fluorescent protein (GFP)-tagged blastospores to the foliage of orchard trees. Individual leaves were resampled by a semidestructive method immediately after in...
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Aureobasidium pullulans is ubiquitous, polymorphic and black fungus producing a variety of important metabolites including enzymes, polysaccharides and single cell protein. It is considered as a probable source for antagonistic activity against a number of phytopathogenic fungi and possible biocontrol agent of post harvest diseases. The present work aims at the antibacterial compounds produced ...
متن کاملGenetic transformation of Aureobasidium pullulans.
Aureobasidium pullulans strain Y117 was transformed to hygromycin resistance using plasmid pDH33, which contains the bacterial hygromycin B phosphotransferase gene (hph) fused to promoter elements of the Aspergillus niger glucoamylase gene (glaA). Southern hybridizations of transformants revealed multiple, integrated copies of the vector. The glaA promoter was not induced by starch in A. pullul...
متن کاملExpression of green fluorescent protein in Aureobasidium pullulans and quantification of the fungus on leaf surfaces.
A red-shifted, mutated form of the jelly-fish green fluorescent protein (GFP) under control of a TEF promoter was expressed at high levels in the filamentous fungus Aureobasidium pullulans. In the three transformants studied, all morphotypes of the fungus, including pigmented chlamydospores, expressed GFP and fluoresced brightly. Confocal microscopy showed that the intra-cellular distribution o...
متن کاملControl of postharvest decay of apple fruit by Aureobasidium pullulans and induction of defense responses
The biocontrol activity of Aureobasidium pullulans on decay of apple fruit caused by Botrytis cinerea and Penicillium expansum, and its ability to induce biochemical defense responses in apple tissue, were investigated. In apple wounds, A. pullulans multiplied rapidly and controlled decay caused by either B. cinerea or P. expansum. At the end of the storage period, A. pullulans reduced the inci...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2003
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.69.8.4892-4900.2003